ACTA Oral Biochemistry
Salivary Research
Laboratory Services
The Oral Biochemistry lab facilitates a broad variety of biochemical technologies:
General protein biochemistry
- Peptide synthesis (linear, cyclization, conjugation)
- Click chemistry
- Protein purification
- Maldi
- SDS page
- Western- and dotblot
- Total Protein measurement
- HPLC
- Mucin isolation by ultracentrifugation
- Phage display
Salivary diagnostics; detection and activity
- Detection of mucins MUC5B and MUC7, albumin, lactoferrin, S-cystatin, secretory Immunoglobulin A, caffeine and steroid hormones in saliva
- Activity of amylase, chitinase, proteases and lysozyme
Antibacterial activity
- Colony counting
- MIC assays
- Propidium Iodide detection
- Anti-biofilm activity
- bacterial aggregation
- ATP, AMP, ADP and NAD(H) determination
Protective functions of saliva
- Salivary buffer capacity
- Salivary flow
- Erosion on hard tissue
- Visco-elastic measurements
- Cell culturing
- Wound healing models (in vitro)